Use of Nichrome wire in the Surface possible counts

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Use of Nichrome wire in the Surface possible counts

The surface possible counting technique demonstrates the use of nichrome wire loop with an outer diameter of 5mm is splashed over a dried platter. The loop grips 1/35 ml of peptone liquid. When it is kept in parallel W5 ml pipettes that provided 1/50 mm of drips, it provided reproducible outcomes not at all lesser to those got from the 50 dripping pipettes.

The preface of surfacing technique of miles for the possible organisms with the pipettes offering 0-2 ml drips gave the mechanism of easier calculation as compare to the pour plate technique.The target of the present thesis is to show a simple formula of making possible counts which is faster since it doesn't need the dropping pipettes and seems to be equivalent to standard methods.

This method uses the loops of nichrome wire to disperse over a prescribed volume of concentrated bacterial suspension on the arid agar or blood agar platters. The Nichrome wire of 0.5mm thickness is used. The immunized wire with length of 13cm or more was used to enable the holder to not to come in contact of the internal dilution vessel. The largest loop that could grip the drip without let it fall due to effect of gravity. The loops of nichrome wire with an outer diameter of 6mm were attempted first for this experiment but they were quite wider so they were substituted by loops of 5 mm width. The diameter was confirmed using measuring magnifier to ensure the internal diameter of 4mm and outer of 5mm.

The piston of injection of 1ml volume was having the diameter of 4mm. Little black piece at the nozzle of the piston eradicated and couple of pilers supported to wind the nichrome wire surrounding the piston. Either a nail or metal stick of 4mm in diameter could also work. Certain magnifier was used with scale of 20mm length divided in 1mm sections though the microscope eyepiece with a micrometer disc having a scale 10mm long could use in the process. However in actual only 3 loops were obtained for demonstration in 1974 because at that the 3^rd loop was used and initial two were kept on cold.

In the preparations of dilute solutions, 4-5 ml volume of solvents chiefly the peptone liquid was poured in the test tube of 150 x 15 mm size.Tenfold intensity of the peptone liquid culture of E coli was prepared. The volume between 0 - 5 mm was used in different tubes while using different sterile straight sided one ml releasing pipettes that were graduated to the edge such that the liquid was absorbed upper and lower side half of the length of pipettes so that the internal surface can be saturated by bacteria that was moving to adhere there and the bacterial accumulations were split.

After the furthermore steps of process the inoculating wire was removed cautiously without contacting the walls of test tube and 834 surface possible counts with the loops of nichrome wire streaked together with the arid platter in the section made especially for it.

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